2024 Prohead core protein protease

Prohead core protein protease

Author: ttgk

August undefined, 2024

WebBacteriophage DNA packaging results from an ATP-driven translocation of concatemeric DNA into the prohead by the phage terminase complexed with the portal vertex dodecamer of the prohead. Functional domains of the bacteriophage T4 terminase and portal gene 20 product (gp20) were determined by mutant analysis and sequence localization within the … WebThe cells were then stained with either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; dashed line histogram) or Alexa Fluor™ 488 Mouse Anti-HCV Core Protein antibody (Cat. No. 569372/569373; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing HCV Core Protein expression (or Ig Isotype control ...

Molecular architecture of the prolate head of bacteriophage T4

Webnome into a preformed capsid shell, or prohead, by means of a powerful molecular motor [1,2]. The DNA-packaging motor is situated at a unique 5-fold vertex of the prohead and is a complex assembly of multiple components. At the core of the motor is the dodecameric head-tail connector, gene product 10 (gp10). Associated with the connector is a ... WebApr 7, 2004 · The prohead contains an internal core made up of the major core protein, gp22, the minor core proteins, gp alt, a serine-type protease, gp21, and other internal … current temperature in manchester

Common Evolutionary Origin of Procapsid Proteases, …

WebProhead core protein protease (EC 3.4.-.-) (Protein Gp21) PCPP_BPT4. Enterobacteria phage T4 (Bacteriophage T4) reference strain. 5JBL. ... Capsid assembly scaffolding protein (Gene product 22) (gp22) (Head morphogenesis protein) (Major prohead-scaffolding core protein Gp22) (Scaffold protein) SCAF_BPB03. Bacillus phage B103 (Bacteriophage B103) WebDuring maturation, all but one of the prohead proteins are proteolytically processed by a phage-coded protease which is formed by autocatalytic cleavage of the product of gene 21 (gp21). Protease gp21 has been tentatively located in the center of the prohead core. Features Showing features for site. Expand table GO annotations Expand table WebFeb 1, 2009 · Based on amino acid sequences of the major capsid protein, KSP90 formed a new branch with a Stenotrophomonas maltophilia phage, Smp14, in the T4-type phage phylogeny. Both Smp14 and phiEco32 have been reported as potential therapeutic phages. charnwood borough council cabinet

Analysis of capsid portal protein and terminase functional …

WebThe prohead is composed of the scaffold protein gp7, the capsid protein gp8, the portal protein gp10, and the dispensable head fiber protein gp8.5. Our objective was to elucidate … Web301 Moved Permanently. nginx current temperature in mannheim germanyWebJul 1, 2003 · Nature Structural Biology. Three-dimensional structures of the double-stranded DNA bacteriophage φ29 scaffolding protein (gp7) before and after prohead assembly have been determined at resolutions of 2.2 and 2.8 Å, respectively. Both structures are dimers that resemble arrows, with a four-helix bundle composing the arrowhead and a coiled coil ... current temperature in manassas va

"Web1 day ago · Argonaute (Ago) proteins are found in all three domains of life. The best-characterized group is eukaryotic Argonautes (eAgos). Being the structural core of RNA interference machinery, they use ... " - Prohead core protein protease

Prohead core protein protease

EHR62637.1 protein (Saccharomonospora cyanea) - STRING …

WebJan 1, 2011 · The latter suggests these proteins are processed by a T4-like prohead morphogenic protease, and are therefore head proteins. Overall, in these phages, there are enough different proteins to account for the capsid shell (including vertex and portal structures) as well as protein mass to account for the inner body structure. WebThe phage T4 head assembly pathway produces a complex prohead consisting of six essential proteins and at least seven nonessential proteins. 4 The T4 prohead maturation protease degrades the scaffolding core into …

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WebMay 3, 1996 · The purified proteins reacted in the presence of polyethylene glycol or dextran to produce prohead-like capsid shells and also polycapsids consisting primarily of head protein, similar to the polycapsids observed after infection by T7 mutants lacking connector or core proteins. WebTwin-arginine protein translocation systems (Tat) translocate fully folded and co-factor-containing proteins across biological membranes. In this review, we focus on the Tat pathway of Gram-positive bacteria. The minimal Tat pathway is composed of two components, namely a TatA and TatC pair, which a … Twin-Arginine Protein Translocation

WebImmunolabeling confirmed the structural presence of the main core protein in both structures. Gel electrophoresis of reversibly cross-linked cores revealed the essential … WebLegend. Settings. Analysis

WebProhead core protein protease · Gene: 21 · Enterobacteria phage T4 (Bacteriophage T4) · EC:3.4.-.- · 212 amino acids · Evidence at protein level · Annotation score: 3/5 #Hydrolase #Protease #Viral capsid assembly #Viral capsid maturation #Viral release from host cell

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Web1 day ago · ORF130-encoded proteins were found to be comparable to the TrudiRoth phage's prohead protease. The morphogenesis of phages begins with the creation of the procapsid structure (also known as the prohead). During capsid development, the protease found in the prohead is activated, destroying the inner core and making room for the … charnwood borough council car parksWebThis study was designed to analyse IgE and its related phenomena in bullous pemphigoid (BP). We analysed 17 BP sera by indirect immunofluorescence (IIF) and immunoblotting (IB) using a monoclonal antibody to IgE. In addition, inflammatory cells in lesional skin from 11 patients with BP were analysed by the alkaline phosphatase-anti-alkaline phosphatase … charnwood borough council bulky wasteWebClass III alcohol dehydrogenase (chi chi-ADH) from human liver binds both ethanol and acetaldehyde so poorly that their Km values cannot be determined, even at ethanol concentrations up to 3 M However, long-chain carboxylates, eg, pentanoate, octanoate, deoxycholate, and other anions, substantially enhance the binding of ethanol and other … current temperature in lynnwood washingtonWebAes012_158 prohead core protein and protease [] Gene ID: 15041397, updated on 2-Aug-2024. Summary Other designations. prohead core protein and protease ... charnwood borough council change of addressWebMay 25, 2024 · The prohead contains an internal core which consists of the major core protein, gp22, the minor core proteins gpalt, a serine-type protease gp21, and other internal proteins . The major capsid protein gp23 and the minor capsid protein gp24 are surrounded by scaffolding core and assembled together [ 24 ]. charnwood borough council cilWebOrgain Organic Unflavored Vegan Protein Powder, Natural Unsweetened - 21g of Plant Based Protein, Non Dairy, Gluten Free, No Sugar Added, Soy Free, Non-GMO, 1.59 lb … current temperature in marrakechWebApr 1, 1984 · Immunolabeling confirmed the structural presence of the main core protein in both structures. Gel electrophoresis of reversibly cross-linked cores revealed the essential head proteins gp22, gp67 ... current temperature in markham